Journal: International Journal of Molecular Sciences

Article Title: Canonical and Non-Canonical Localization of Tight Junction Proteins during Early Murine Cranial Development

doi: 10.3390/ijms25031426

Figure Lengend Snippet: ZO-1 shows the strongest expression on the apical surface of the mouse neuroepithelium. ( A ): Confocal images show a representative coronal section of mouse cranial neural folds with immunofluorescence co-staining detecting ZO-1 (yellow) and integrin alpha 4 (ITGA4, magenta) at embryonic stage E 8.5 (9 somites, 9s). The overlay image of all channels (merge) is shown at the top of panel ( A ), followed below by the individual channel images, showing the signals for ITGA4 (magenta) and ZO-1 (yellow). Nuclei are stained with DAPI (cyan). Scale bar: 50 µm. Immunofluorescence staining for ITGA4 was performed to label the basolateral cellular domains. ZO-1 showed the strongest signals in the neuroectoderm (NE) and less strong signals in the non-neuronal ectoderm (NNE) and the mesenchymal cells, underlying the neuronal ectoderm. ( A′ ): Higher magnification of boxed area in ( A ). The overlay image of all channels is shown at the top of panel ( A′ ), followed below by the individual channel image, showing the signals for ZO-1 (yellow). Scale bar: 5 µm. ZO-1 was localized to the apical cell–cell junctions. ( B ): Confocal images show representative coronal section of mouse cranial neural folds with immunofluorescence co-staining detecting ZO-1 (yellow) and ITGA4 (magenta) at embryonic stage E 9.0 (13 somites, 13s). The overlay image of all channels is shown at the left of panel ( B ), followed to the right by the individual channel images, showing the signals for ITGA4 (magenta) and ZO-1 (yellow). Nuclei are stained with DAPI (cyan). Scale bar: 50 µm. ZO-1 showed the strongest signals in the neuroectoderm (NE) and less strong signals in the non-neuronal ectoderm (NNE) and mesenchymal cells. ( C ): E 8.0 whole-mount mouse embryos (6 somites, 6s) were immunofluorescence co-labelled for ZO-1 (yellow) and ITGA4 (magenta). The overlay image of both channels is shown at the top of panel ( C ), followed below by the individual channel image showing the signals for ZO-1 (yellow). The frontal view on the whole-mount forebrains, imaged using confocal microscopy, is shown. Scale bar: 50 µm. ( C′ ): Magnification of boxed area indicated in ( C ) with merged-channel image at the top, showing ITGA4 (magenta) and ZO-1 (yellow) signals and ZO-1 single-channel image below. Scale bar: 10 µm.

Article Snippet: Rabbit ITGA4 , Cusabio, Houston TX, USA, CSB-PA011867LA01HU , 1:200.

Techniques: Expressing, Immunofluorescence, Staining, Confocal Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Canonical and Non-Canonical Localization of Tight Junction Proteins during Early Murine Cranial Development

doi: 10.3390/ijms25031426

Figure Lengend Snippet: List of antibodies.

Article Snippet: Rabbit ITGA4 , Cusabio, Houston TX, USA, CSB-PA011867LA01HU , 1:200.

Techniques:

Journal: Experimental gerontology

Article Title: Col1a1 mediates the focal adhesion pathway affecting hearing in miR-29a mouse model by RNA-seq analysis.

doi: 10.1016/j.exger.2023.112349

Figure Lengend Snippet: Fig. 7. ITGA4 and PIK3R3 are expressed in the cochlea. (A) The levels of ITGA4 and PIK3R3 proteins in the cochlea of 2-month miR-29a+/+ mice and miR-29a−/−

Article Snippet: The primary antibodies used in this study are COL1A1 (1:50, Santa, sc-293182), ITGA4 (1:50, Santa, sc-376334), ITGB3 (1:50, Santa, sc-365679), PIK3R3 (1:50, ABclonal, A17112) and PTK2 (1:50, Santa, sc-1688).

Techniques:

Journal: Frontiers in Nutrition

Article Title: Prevention of high-fat/high-sugar diet-induced type 2 diabetes mellitus-associated non-alcoholic fatty liver disease in rats with fermented and raw Rosa roxburghii Tratt (Cili) juice

doi: 10.3389/fnut.2025.1584551

Figure Lengend Snippet: Expression analyses of transcription or protein levels of selected genes. (A) Expression analyses of selected genes by qRT–PCR. The data represent the means ± SDs from six biological replicates with three technical replicates. (B–D) FCJ/RCJ upregulated ABCC3, IDI1, and APOA2 expression in the liver. FCJ and RCJ significantly upregulated hepatic ABCC3 (B) and IDI1 (C) expression, and RCJ significantly upregulated hepatic APOA2 (D) expression in T2DM rats. The data represent the means ± SDs from three biological replicates with three technical replicates. * p < 0.05, *** p < 0.001, **** p < 0.0001.

Article Snippet: The western blotting antibodies used included APOA2 (BM5624, BOSTER, China), IDI1 (A07892-1, BOSTER, China), ABCC3 (DF3874, Affinity, United States), β-tubulin (Solarbio, China), goat anti-rabbit (BS13278, Bioworld, United States), and goat anti-mouse (BS12478, Bioworld, United States) antibodies.

Techniques: Expressing, Quantitative RT-PCR